| Proteins (Sparassis crispa) identified by 2-DGE and N-terminal amino acid sequencing | |||||||||
| The table lists indicated spot numbers, amino acid sequences, scores, accesion numbers, protein names, identity/similality, theoretical mass (kDa), theoretical pH, organism and function left to right. | |||||||||
| Spot Number | Amino Acid Sequence | Score | Accession Number | Protein Name | Identity /Similarlity | Analytical MW | Theoretical pI | Organism | Functional Category |
| 1 | gefsddsdqagaydq | 74 | A1CE10 | Phosphoglycerate mutase* | 73 / 87 | 12190.12 | 4.81 | Fungi | Carbohydrate transport and metabolism |
| 2 | sntgvvaatv | No significant hits found | |||||||
| 3 | sptvxvndvvpsgtftyvpy | 74 | Q2PCV2 | Laccase* | 69 / 81 | 5404.94 | 6.26 | Fungi | Secondary metabolites biosynthesis, transport, and catabolism |
| 4 | sxraaxgsgavnags | No significant hits found | |||||||
| 5 | raakgsgavnagstqsfvgnlseln | No significant hits found | |||||||
| 6 | rgkggsgvnnggtqqffv | 65 | Q6ZXX3 | Histone H4 | 53 / 100 | 11382.34 | 11.36 | Fungi | Chromatin structure and dynamics |
| 7 | aplgievadapstaafgv | 74 | Q1IL41 | Hypothetical protein | 53 / 88 | 32116.70 | 5.64 | Bacteria | Unclassified |
| 8 | aplsievadapstiafgv | 61 | Q9FAX2 | DNA gyrase subunit B* | 56 / 78 | 53556.69 | 6.33 | Bacteria | DNA replication, recombination, and repair |
| 9 | sdsiearlhfienln | 56 | P46963 | CTD kinase subunit gamma* | 69 / 92 | 34809.18 | 5.88 | Fungi | Transcription |
| 10 | sdsiearlhfienln | 56 | P46963 | CTD kinase subunit gamma | 69 / 92 | 34809.18 | 5.88 | Fungi | Transcription |
| 11 | sdsiearlhfienln | 56 | P46963 | CTD kinase subunit gamma | 69 / 92 | 34809.18 | 5.88 | Fungi | Transcription |
| 12 | sdsieaqlhfienln | 54 | Q753W3 | Vacuolar sorting protein SNF7* | 64 / 79 | 26685.19 | 4.84 | Fungi | Intracellular trafficking and secretion |
| 13 | speqelaavngrllqssvgdldpvv | 79 | A0V740 | Phosphatidylserine decarboxylase* | 54 / 75 | 30888.40 | 9.09 | Bacteria | Lipid metabolism |
| 14 | speqelaavngrllqssvgd | No significant hits found | |||||||
| 15 | sldvearalyiesldifg | 59 | Q3IYU1 | tRNA pseudouridine synthase B* | 56 / 88 | 32194.57 | 5.20 | Bacteria | Translation, ribosomal structure and biogenesis |
| 16 | rvpketlidqlakay | No significant hits found | |||||||
| 17 | gldklvnvnglqdqmgvgdl | 68 | Q8ZVM9 | Probable exosome complex exonuclease 1* | 56 / 94 | 27148.26 | 6.86 | Bacteria | Amino acid transport and metabolism |
| 18 | rlgkvgnvrgagg | No significant hits found | |||||||
| 19 | spdkltnvnl | No significant hits found | |||||||
| 20 | aapapkapkgvekrd | No significant hits found | |||||||
| 21 | slnmvyrlmf | No significant hits found | |||||||
| 22 | slimqlrltf | No significant hits found | |||||||
| 23 | kldldllvrlrldah | No significant hits found | |||||||
| 24 | kadmvtrvrl | 47 | Q9MUU6 | Chloroplast 30S ribosomal protein S8* | 75 / 100 | 14764.25 | 9.73 | Plant | Translation, ribosomal structure and biogenesis |
| 25 | kqsredlvylaklgr | 64 | Q562H7 | 14-3-3 protein | 92 / 92 | 28815.19 | 4.76 | Fungi | Signal transduction |
| 26 | snpnrkpgqhdlall | No significant hits found | |||||||
| 27 | kpgagengdg | No significant hits found | |||||||
| 28 | kpgvhfmpfddfgld | No significant hits found | |||||||
| 29 | kapegnilggggdrg | No significant hits found | |||||||
| 30 | lkdlqedhagvrlqv | 80 | O14460 | Elongation factor 2 | 73 / 93 | 93230.90 | 6.02 | Fungi | Translation, ribosomal structure and biogenesis |
| 31 | kpqytpanigapgrg | 69 | O57683 | Splicing factor 3B subunit 1* | 57 / 100 | 146214.52 | 6.48 | Insect | RNA processing and modification |
| 32 | N-terminal amino acid blocked | ||||||||
| 33 | N-terminal amino acid blocked | ||||||||
| 34 | asatehtltvrdgln | 53 | Q9B6D8 | Cytochrome c oxidase subunit 3* | 73 / 91 | 30467.04 | 6.32 | Fungi | Energy production and conversion |
| 35 | psakifydsdadlsl | 73 | Q46JF6 | Ketol-acid reductoisomerase* | 85 / 100 | 36309.51 | 5.21 | Bacteria | Amino acid transport and metabolism |
| 36 | slpegytvlsnrdsi | No significant hits found | |||||||
| 37 | slgrygiggfl | No significant hits found | |||||||
| 38 | pnvqrvtexvfqsps | 50 | Q9FNE0 | Putative cytochrome c oxidase polypeptide Vc-4* | 43 / 86 | 7421.61 | 8.03 | Plant | Energy production and conversion |
| 39 | N-terminal amino acid blocked | ||||||||
| 40 | qpgdelrdef | 59 | Q8DJ62 | Urease accessory protein ureE* | 89 / 100 | 16285.70 | 6.49 | Bacteria | Amino acid transport and metabolism |
| 41 | qpgydgvlaryrlgp | 64 | Q73V62 | UPF0061 protein MAP 3154 | 67 / 87 | 53324.47 | 5.07 | Bacteria | Unclassified |
| 42 | spgnalevgy | 54 | P22553 | S-locus-specific glycoprotein BS29-2 precursor* | 70 / 100 | 49543.04 | 8.48 | Plant | Sporulation |
| 43 | qleydynghq | No significant hits found | |||||||
| 44 | lqtaadstqleda | 52 | P48465 | Actin | 82 / 100 | 41736.68 | 5.17 | Bacteria | Cytoskeleton |
| 45 | rigyngytnrfggrf | No significant hits found | |||||||
| 46 | N-terminal amino acid blocked | ||||||||
| 47 | rvtglklgavailrv | 57 | Q8NSS8 | Hypothetical transport protein Cgl0590/cg0683 | 67 / 87 | 55665.64 | 5.78 | Bacteria | Unclassified |
| 48 | kgnggllgqnadlgr | 64 | P43852 | Bifunctional purine biosynthesis protein purH* | 83 / 92 | 58349.59 | 5.75 | Bacteria | Nucleotide transport and metabolism |
| 49 | qiggasgggr | No significant hits found | |||||||
| 50 | mvlveelnvlva | 45 | Q83PY1 | Protein slyX | 64 / 82 | 8184.30 | 4.89 | Bacteria | Unclassified |
| 51 | kgyatpiftmd | No significant hits found | |||||||
| 52 | ateatgtigqvktvigavvd | 86 | Q98EV8 | ATP synthase subunit beta* | 65 / 95 | 50775.77 | 4.97 | Bacteria | Energy production and conversion |
| 53 | slgahgakry | 59 | Q00094 | Gene 73 protein kinase* | 80 / 100 | 106567.73 | 8.80 | Viruses | Signal transduction |
| 54 | pltfsqqfntsv | 52 | Q8CWA2 | Cation efflux system protein cusB precursor* | 67 / 92 | 44409.95 | 5.70 | Bacteria | Cell envelope biogenesis, outer membrane |
| 55 | rtagepydtv | No significant hits found | |||||||
| 56 | paiaeikvpathkti | 53 | Q4WGM4 | Ubiquinol-cytochrome c reductase complex* | 47 / 80 | 14662.88 | 5.55 | Fungi | Energy production and conversion |
| 57 | apqtkllqatgdaay | 51 | P94186 | Mercuric transport protein periplasmic component precursor* | 73 / 91 | 9505.12 | 9.26 | Bacteria | Inorganic ion transport and metabolism |
| 58 | ahkdikfsnegrasi | 70 | Q0H0L2 | Heat shock protein 60 | 71 / 83 | 63039.38 | 5.91 | Fungi | Posttranslational modification, protein turnover, chaperones |
| 59 | slqvaliayqkndyh | 55 | Q7VI68 | L-seryl-tRNA(Sec) selenium transferase* | 53 / 93 | 50580.15 | 8.50 | Bacteria | Translation, ribosomal structure and biogenesis |
| 60 | klgdklgrkg | 67 | Q0K852 | NADH dehydrogenase type2 * | 100 / 100 | 47467.77 | 8.64 | Bacteria | Energy production and conversion |
| 61 | glearvptdsqp | 67 | Q2H0Z4 | Hypothetical protein | 75 / 100 | 51089.66 | 4.91 | Fungi | Unclassified |
| 62 | qteeyfvitlqflhv | 52 | P37485 | Hypothetical protein yybS | 64 / 91 | 34534.02 | 9.65 | Bacteria | Unclassified |
| 63 | spgtlprgkhrgmgs | 77 | Q2LEM4 | Hypothetical protein | 71 / 86 | 25414.61 | 5.89 | Bacteria | Unclassified |
| 64 | sqgeglgtnier | No significant hits found | |||||||
| 65 | atvsegnigaty | No significant hits found | |||||||
| 66 | alvgeggrgt | No significant hits found | |||||||
| 67 | N-terminal amino acid blocked | ||||||||
| 68 | slgrvdllrt | No significant hits found | |||||||
| 69 | sllvvgygth | No significant hits found | |||||||
| 70 | klraelnen | No significant hits found | |||||||
| 71 | klrayrnvlk | 49 | Q6EW16 | Chloroplast 30S ribosomal protein S8* | 88 / 100 | 15447.03 | 11.30 | Plant | Translation, ribosomal structure and biogenesis |
| Asterisks indicate unique proteins identified from S. crispa fruiting body using Tandem MS analysis and Edman sequencing. Commonly identified proteins in S. crispa and H. erinaceum are shown in red letters. | |||||||||